Effects of Fluoranthene, a Polycyclic Ambrosial Hydrocarbon, on cAMP-Dependent Anion Secretion in Animal Airway Epithelia

  The animal respiratory amplitude is consistently apparent to polycyclic ambrosial hydrocarbons (PAHs) through assimilation of atmospheric pollutants. We advised the furnishings of three PAHs (benzo[a]pyrene, anthracene, and fluoranthene) on the airway ion transport, which is capital for lung aegis and accustomed airway function, appliance animal airway epithelia (Calu-3). These three PAHs had no cogent aftereffect on the basal circumlocute accepted (Isc).  However, fluoranthene (1–100 μM) activated in the aciculate alcove potentiated Isc in acknowledgment to cAMP-related agents (isoproterenol, forskolin, and 8-bromo-cAMP). The furnishings of fluoranthene were artless by ellipticine, a PAH receptor antagonist. The fluoranthene-induced modulations of these anion transporters were counteracted by charybdotoxin (ChTx, a hIK-1 approach blocker). Fluoranthene gradually aggrandized the ChTx-sensitive K+ accepted (IK) beyond the basolateral membrane, accompanied by a abiding access in the cytosolic Ca2+concentration ([Ca2+]i).  In the attendance of fluoranthene, however, a abundant beyond hIK-1-dependent IK was articular by the appliance of 8-bromo-cAMP after accessory acclivity of [Ca2+]i.