Development of Dioctadecyldimethylammonium Monoolein Liposomes for Gene Delivery

  The electrostatic abhorrence amid the phosphodiester anionic accuse of nucleic acids (NA) and the negatively-charged headgroups of corpuscle film phospholipids hinders naked NA to charge the claret membrane. Additionally, nucleases present in the beef and in biological fluids enzymatically abase NA, attached their biofunctionality. Although abounding another methods accept been developed to bear NA to cells, factors such as versatility, account and ability accept beat their broadcast use in gene therapy.  Given these limitations, the intracellular supply of abiogenetic actual can alone be accomplished through the use of physical, biological or actinic methods that advance gene admittance into cells. Concrete methods accept about low in vivoapplicability and cover absolute bang of NA into organs of reside animals, micro projectile biolistics with a gene-propelling gun, corpuscle sonication application an accelerated transducer and corpuscle electroporation by acknowledgment to an electric field.  Biological methods await on attenuated or inactivated versions of adenoviruses, lentiviruses and retroviruses, whose deactivated apparatus can be acclimated as gene vectors to access almost top in vivo transfection efficiencies. Viral vectors affectation about important safety, toxicity and immunogenicity issues, which abundantly absolute their use in humans. Actinic methods are based on the use of actinic adjuvants with almost top levels of biocompatibility, such as constructed polymers and cationic liposomes (lipofection). These molecules self-assemble in awful organized structures able of complexing the abiogenetic actual and after absolution it central the cells.  Whereas bacilli about appoint issues of mutagenicity and immunogenicity, polymers such as polyethylenimine (PEI) are accepted to be awful cytotoxic. By exclusion of alternatives, cationic liposomes accept emerged as the carriers of arete for intracellular supply of nucleic acids due to their top versatility, bargain cytotoxicity, and top transfection efficiency.